Objective
The testis determining gene Sry is a key element in the mammalian sex-determination pathway. There is some in vitro evidence that the mouse SRY protein acts as a transcriptional activator. To test this hypothesis in vivo, we will create a repressor allele of SRY in transgenic mice. An active repressor can be generated by linking the transcription repressor domain of the Drosophila gen Engrailed to the binding domain of SRY. If this causes sex reversal in the transgenic mice, then SRY probably acts as a transcriptional activator. The reverse experiment, adding a strong activator domai to SRY, should prove this.
The cellular distribution of SRY has never been determined in vivo, because good antibodies are not yet available. We propose to create an epitope tagged SRY protein in transgenic mice. Using antibodies against the epitope, we will be able to detect the protein in vivo. The existence of an epitope tagged SRY protein will also aid in the search for downstream target genes.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
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Programme(s)
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
EH4 2XU Edinburgh
United Kingdom
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