Objective
In E. coli, most transcription activators work by making contacts with either the a subunit of RNA polymerase (Class-l transcription activators) or with the cs factor (Class-IU). The Ada protein, a transcriptional activator for a set of genes involved in repair of DNA alkylation damage, has been identified as a Class-l activator, since deletions in the C-terminal domain of the a subunit severely impair Ada activation at the ada promoter. We have shown that the C-terminal domain of a is in fact responsible for binding of RNA polymerase to the promoter region of the ada and the aidB genes.
This observation suggests that the role played by the a subunit of RNA polymerase at these promoters is to interact with the promoter DNA rather than with the transcriptional activator Ada. This possibility would suggest a novel mechanism for RNA polymerase interaction with positively controlled promoters.
In this proposal, we outline a series of experiments to determine if a-mediated binding of RNA polymerase to the ada and aidB promoters is necessary for Ada-activated transcription.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences genetics RNA
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Programme(s)
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
B15 2TT Birmingham
United Kingdom
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