A new model for rna polymerase/promoter interaction in escherichia coli

Project Information

Grant agreement ID: FMBI960947

Project closed

Start date 1 January 1997

End date 31 December 1998

Funded under

• Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998

Total cost No data

EU contribution No data

Coordinated by

University of Birmingham
United Kingdom

Objective

In E. coli, most transcription activators work by making contacts with either the a subunit of RNA polymerase (Class-l transcription activators) or with the cs factor (Class-IU). The Ada protein, a transcriptional activator for a set of genes involved in repair of DNA alkylation damage, has been identified as a Class-l activator, since deletions in the C-terminal domain of the a subunit severely impair Ada activation at the ada promoter. We have shown that the C-terminal domain of a is in fact responsible for binding of RNA polymerase to the promoter region of the ada and the aidB genes.
This observation suggests that the role played by the a subunit of RNA polymerase at these promoters is to interact with the promoter DNA rather than with the transcriptional activator Ada. This possibility would suggest a novel mechanism for RNA polymerase interaction with positively controlled promoters.
In this proposal, we outline a series of experiments to determine if a-mediated binding of RNA polymerase to the ada and aidB promoters is necessary for Ada-activated transcription.

Fields of science (EuroSciVoc)

CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.

• natural sciences › biological sciences › genetics › DNA
• natural sciences › biological sciences › biochemistry › biomolecules › proteins
• natural sciences › biological sciences › genetics › RNA

You need to log in or register to use this function

Programme(s)

• FP4-TMR - Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998

Topic(s)

• 0302 - Post-doctoral research training grants
• TL02 - Molecular Biology and Biochemistry

Call for proposal

Data not available

Funding Scheme

RGI - Research grants (individual fellowships)

Coordinator

University of Birmingham

• EU contribution: No data
• Address: Edgbaston ; B15 2TT Birmingham ; United Kingdom
• Total cost: No data

Participants (1)

Not available

Italy

• EU contribution: No data
• Total cost: No data