Objective
My pilot studies suggest that (I) Schwann cells undergo programmed cell death in vitro when cultured at low cell density; (II) that this has not been noticed previously because Schwann cells secrete autocrine survival factor(s) that prevent their death in routine medium / high density cultures, and (III) that neuregulins and neurotrophins might support Schwann cell survival. In this project I plan to confirm, analyse and extend these observations.
Firstly, the mechanisms of cell death will be further studied using in situ hybridization and ultrastructural studies, and the death will be quantified with respect to cell density, time in vitro and type of culture substrate. Secondly, growth factors will be screened for their ability to block Schwann cell death, with particular emphasis on neuregulins and neurotrophins. Thirdly, I will aim to identify the autocrine survival promoting activity present in Schwann cell conditioned medium using blocking antibodies and ligand trap methods. Fourthly, the developmental expression of relevant receptors and their regulation will be investigated. Lastly, if time allows I will seek to interfere with the expression of functional receptors by overexpression of dominant negative mutations.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
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Programme(s)
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
WC1E 6BT London
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.