Objective Fluorescence resonance energy transfer (FRET) techniques offer a new and unique approach for evaluating molecular interactions within cells, and have led to breakthroughs in our understanding of DNA structures. The objective of the proposed research is to extend the current FRET techniques to offer the capability for continuous observation at a high temporal resolution. The innovative aspect of the method described in this proposal is that it will minimize the probability of irreversible destruction of the donor or acceptor, which cannot be achieved with the current photobleaching FRET techniques. The proposed concept will be implemented on a confocal microscope imaging system allowing the mapping of energy transfer efficiencies on a pixel-by-pixel basis. The method will be used to study nucleic acid interactions, and protein-nucleic-acid interactions in an interdisciplinary effort with other scientists in the host laboratory. Fields of science natural sciencesbiological sciencesbiochemistrybiomoleculesnucleic acidsnatural sciencesbiological sciencesgeneticsDNAnatural sciencesphysical sciencesopticsmicroscopyconfocal microscopy Programme(s) FP4-TMR - Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998 Topic(s) 0302 - Post-doctoral research training grants TP14 - Biophysics Call for proposal Data not available Funding Scheme RGI - Research grants (individual fellowships) Coordinator MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V. Address 11,am fassberg 11 37077 Göttingen - nikolausberg Germany See on map EU contribution € 0,00 Participants (1) Sort alphabetically Sort by EU Contribution Expand all Collapse all Not available Netherlands EU contribution € 0,00 Address See on map
