Skip to main content
European Commission logo
English English
CORDIS - EU research results
CORDIS
Content archived on 2024-04-30

Messenger rna sequences responsible for selective sorting and transport of glycine receptor subunit mrnas in rat and mouse central neurones

Objective



From oocytes to neurones and from Drosophila to vertebrates, intracellular targeting and localization of molecules contribute to regulation of gene expression.
In the past year I performed light (lm) and electron (em) microscopic nonisotopic in situ hybridization (ISH) studies to investigate the regional and subcellular distribution of glycine receptor (GlyR) subunit mRNAs in rat and mouse CNS neurones. I found that alpha and beta subunit mRNAs have somatodendritic and somatic localizations, respectively. Studies of other nonneuronal cell types suggest that the differential intracellular distribution of mRNAs depends on signal sequences in the 3'untranslated region (UTR). I will construct, express in neuronal cell cultures, and visualize by lm and em ISH tagged-chimeric GlyR subunit cDNAs, containing or lacking normal or mutated 3'UTR. This will allow identification of the mRNA targeting sequences which distinguish and determine, by interaction with the trafficking machinery, the different intracellular distributions of GlyR subunit transcripts.

Call for proposal

Data not available

Coordinator

Ecole Normale Supérieure
EU contribution
No data
Address
46,Rue d'Ulm 46
75230 PARIS
France

See on map

Total cost
No data

Participants (1)