Nuclear receptor co-regulator complexes - assembly, specificity and role in transcription

Project Information

Grant agreement ID: FMBI961269

Project closed

Start date 1 September 1996

End date 31 August 1999

Funded under

• Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998

Total cost No data

EU contribution No data

Coordinated by

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
France

Objective

Nuclear receptors function as DNA-bound ligand-inducible transcription factors that influence differentiation, proliferation, and development via regulation of target genes. A number of co-regulators (or: "mediators", "adaptors", "transcription intermediary factors (TlFs)") have been recently identified in the host institute and other laboratories which form complexes with nuclear receptors in a ligand dependent manner and are required for transmission of the pleiotropic signals to the basal transcription machinery and chromatin. However, the identity of complexes comprising nuclear receptors and cGregulators and their modes of action are yet unknown.
My goal is to (i) in vitro reassemble such complexes on biotinylated DNA using purified proteins and/or different nuclear extracts; (ii) characterise these complexes by using receptor mutants, artificial ligands and other means; (iii) test their activity in in vitro transcription assays using immobilised target promoters as templates; and (iv) analyse the effect of in vitro assembled chromatin on transcription from such promoters.
This study will allow us to understand the functioning of nuclear receptor co-regulator complexes in transcription, and to unravel the discrepancies between transcriptional regulation in vitro and in vivo.

Fields of science (EuroSciVoc)

CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.

• natural sciences › biological sciences › genetics › DNA
• natural sciences › biological sciences › biochemistry › biomolecules › proteins

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Programme(s)

• FP4-TMR - Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998

Topic(s)

• 0302 - Post-doctoral research training grants
• TL02 - Molecular Biology and Biochemistry

Call for proposal

Data not available

Funding Scheme

RGI - Research grants (individual fellowships)

Coordinator

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE

• EU contribution: No data
• Address: Rue Laurent Fries 1 ; 67404 ILLKIRCH GRAFFENSTADEN ; France
• Total cost: No data

Participants (1)

Not available

Germany

• EU contribution: No data
• Total cost: No data