Objective
We have used an insulinoma cell line, RIN-B, which expresses most of the putative IDDM autoantigens (GAD 65 and 67, insulin, CPH, 38 kDa insulin granule protein). ICA positive sera from IDDM patients are positive with RIN-B cells and it has been shown that T cells from IDDM patients have specific reactivity to RIN-B extracts which can specifically induce the proliferation of a T cell clone specific for the 38kDa of islet granules. The objective of this project is the study of the capacity of an endocrine epithelial cell to process and present target autoantigens to T cells. We are using the RIN cell line as the basis to construct a panel of transfectants expressing different combinations of HLA molecules DR4 or DR3, human B7 as a co-stimulatory molecule and the invariant chain (Ii) and HLA-DM so the class II molecules expressed are internally transported and loaded with peptide in a way similar to a normal APC. This panel of transfectants is being used to study: 1) the processing capacity of the cells as asessed by the analysis of peptides bound to HLA-DR4 or DR3 in the different transfectants and 2) their capacity to present antigen by inducing a primary allogeneic response from PBL or to activate the response of alloreactive, antigen specific and autoreactive T cell clones.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
08916 Badalona
Spain
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.