Nmr studies of the collagen binding domain of fibronectin

Project Information

Grant agreement ID: FMBI961391

Project closed

Start date 1 January 1997

End date 31 December 1998

Funded under

• Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998

Total cost No data

EU contribution No data

Coordinated by

THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD
United Kingdom

Objective

The collagen-binding domain of fibronectin contains four Fnl and two Fn2 modules. It is not yet understood which of these modules interact with collagen. The primary aim of the project is to determine this interaction with a strong candidate for the collagen-binding activity: the pair of modules 6Fnl-lFn2. The combination of NMR spectroscopy and ligand binding studies is extremely powerful to investigate protein-protein interactions in their native states. The first part of the project includes the expression, purification and determination of the tertiary solution structure of 6Fnl-lFn2. The second stage involves the study of the interactions between the pair of modules and the 20 residue fibronectin-binding site of collagen using BIAcore, NMR spectroscopy and affinity chromatography.
6Fnl-lFn2 has been expressed, purified and is now being sequentially assigned using 2D and 3D NMR experiments. Structure calculation and binding studies will commence soon.

Fields of science

Not validated

• natural sciences › biological sciences › biochemistry › biomolecules › proteins › proteomics
• natural sciences › physical sciences › optics › spectroscopy › absorption spectroscopy

Programme(s)

• FP4-TMR - Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998

Topic(s)

• 0302 - Post-doctoral research training grants
• TL02 - Molecular Biology and Biochemistry

Call for proposal

Data not available

Funding Scheme

RGI - Research grants (individual fellowships)

Coordinator

THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD

• EU contribution: No data
• Address: SOUTH PARKS ROAD ; OX1 3QU OXFORD ; United Kingdom
• Total cost: No data

Participants (1)

Not available

France

• EU contribution: No data
• Total cost: No data