Skip to main content
European Commission logo print header

Reaction products of chlorohydroxyfuranones and base units in dna

Objectif



It is well recognized that extracts of most drinking water disinfected with chlorine show mutagenic activity in the Ames Salmonella assay. Chlorohydroxyfuranones (CHFs), and specially the compound MX (3-chloro-4-(dichloromethyl)-5-hydroxy2(5H)-furanone), account for a significant part of this mutagenicity.
The objective of the present work is to investigate the formation and identify adducts produced in the reaction of DNA with CHFs. The previously published and on going studies on adducts identification by Kronberg et al. provide clues on adducts likely to be formed in DNA. Identification of the adducts are based on comparison of HPLC retention times, UV- and mass spectra with those of known adducts. 1H- and 13cNMR are used to identify unknown adducts. We will also develop a GC-MS method for adduct identification (complimentary to the HPLC-UV method). If sensitive enough, such a method could enable analysis of adducts in various tissues of test animals exposed to CHFs.

Appel à propositions

Data not available

Coordinateur

Aabo Akademi University
Contribution de l’UE
Aucune donnée
Adresse
1,Akademigatan
20500 Abo
Finlande

Voir sur la carte

Coût total
Aucune donnée

Participants (1)