Objective
Rab proteins are small GTPases that regulate different exocytic and endocytic transport processes. Rab5 is involved in endocytosis and fusion between early endosomes and functions by recruiting its effector, rabaptin-5, from cytosol to early endosomes in a GTP-dependent manner. The function of rabaptin-5, however, is unknown.
The aim of this proposal is to study the role of rabaptin-5. Preliminary experiments indicate that rabaptin-5 belongs to a multiprotein complex. To identify the molecules interacting with rabaptin-5 and study their function, the complex will be isolated by chromatographic techniques and its constituents molecularly cloned. The assembly of the complex will be investigated biochemically and its functional role will be studied both in vitro in a cell-free early endosome fusion assay as well as in vivo in transfected cells. The two-hybrid system will also be used as an alternative approach to identify rabaptin-5 interacting partners. Our findings will help elucidating the molecular principles underlying the docking and fusion process in membrane transport.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
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Programme(s)
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
69117 Heidelberg
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.