Research objectives and content
The general purpose of this study is to analyse how cells in dorsal and ventral regions of floral meristems in Antirrhinum become progressively committed to form organ with distinct shapes and properties. We intend to search for candidate target genes that are specifically expressed in dorsal or ventral organs of the flower by techniques of subtraction of cDNA. Once isolated, a general method for inactivating the cloned genes will be developed by exploiting a mutagenised seeds to screen for transposon insertions within these genes of interest by PCR. A correlation between these genes and their mutant phenotypes will allow a deeper understanding of their molecular function in the plant. In addition, the
transposon-mutagenised seeds will be used for phenotypic screens to identify further genes involved in dorsoventrality through their effects on flower shape
Training content (objective, benefit and expected impact)
The laboratory of Dr. E. Coen is one of the leading laboratories in the research of flower development and so will provide an excellent and stimulating environment in which to carry out this project. This training, will give me important experience in developmental biology of plants that, together with my present experience will help-me to establish my own research in the future.
Links with industry / industrial relevance (22)