Objective
The aim of the proposed work is to study the folding of the B 1 domain of the immunoglobulin G binding protein from Streptococcus using a variety of experimental techniques, including stopped-flow, fluorescence, circular dicroism, urea, GuHCI and thermal denaturation, as well as site-directed mutagenesis. The B1 domain has the qualities to make it an ideal system for studying the folding process. Recently the E. coli strains super-expressing this domain have been designed by Dr. Serrano's group. This opens the possibility to start GB1 folding studies using all the techniques mentioned. In addition, we can introduce mutations into the domain surface to increase the stability of secondary structure elements. This influence can be a priori estimated by empirical algorithms developed by the group.
It would be most interesting to compare the observed experimental energetic changes with those predicted by theoretical calculations in order to investigate whether it is possible to use these algorithms for a rational increase in protein stability. The increase in protein stability is a major goal in protein engineering because of its potential industrial and pharmacological applications.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins protein folding
- natural sciences biological sciences genetics mutation
- natural sciences mathematics applied mathematics mathematical model
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Programme(s)
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Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
HEIDELBERG
Germany
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