Objective
Research objectives and content
This project is designed to investigate the events induced by the CaMKII phosphorylation cascade in order to activate the ubiquitin-dependent cyclin B degradation during meiosis. The project focus his attention to answer two main questions: 1) Is CaMKII involved in the formation of cyclin B-ubiquitin intermediates? 2) Does CaMKII phosphorylate the APC complex?
1) Role of CaMKII on the formation of cyclin B-ubiquitin complexes. Xenopus CSF extracts will be preincubated with 125I-labeled ubiquitin plus human cyclin B1 during 5 min. Subsequently, Ca2+ will be added to the extracts in order to activate cyclin degradation. The results will be checked by SDS-PAGE electrophoresis followed by autoradiography as a function of time elapsed from Ca2+ addition.
2) Role of CaMKII in the phosphorylation of subunits of the APC complex APC complex will be purified from Xenopus CSF extracts as previously described. Once purified, endogenous phosphorylation assays will be performed either in the presence of purified normal CaMKII enzyme or of constitutively active CaMKII (1-290) peptide together with 32P-yATP. The phosphorylated pattern will be visualized by SDS-PAGE electrophoresis and autoradiography. Localization of the different proteins of APC complex will be checked by coomassie blue staining and western blot assays with specific antibodies.
Training content (objective, benefit and expected impact)
During the last four years I have centred my interest on two main subjects, one of them related with cell cycle control and the other one with the study of nuclear calmodulin functions. Dr. Dorie was the first that described an important role of calmodulin in the cell cycle control, and moreover, his studies were developed in Xenopus oocytes, one of the most useful models to study cell cycle control. This two facts make me interest in the possibility to develop a postdoctoral stay in Dr. Dorie's laboratory. This stay would allow me by one hand to get fresh knowledge on Xenopus oocytes model and on the other hand to integrate the two fields which I have been involved with. Moreover, the results of the present project could considerably contribute to the understanding of cell cycle control either in physiologic or in the pathologic states as cancer. Links with industry/industrial relevance (22)
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences clinical medicine oncology
- natural sciences chemical sciences electrochemistry electrophoresis
- natural sciences biological sciences genetics heredity
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
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Programme(s)
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
34033 Montpellier
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.