Objective
Research objectives and content
Colonization is thought to play an important role in determining probiotic properties of lactobacilli. The relationship between structure and function of two Lactobacillus adhesion proteins will be studied to increase our insight in mechanisms of colonization and to contribute in designing strains suited as vehicles mucosal immunization programmes. The first part of the project, determination of regions of S-layer proteins involved in recognition of receptors on the extracellular matrix, involves the construction and analysis of hybrid S-proteins comprising regions of L. crispatus (adhesion positive) and L. acidophilus ( adhesion negative) S-proteins.
Secondly, the structure-function relationship will be determined of an adhesion factor of L. fermentum showing strong similarity to virulence factors from pathogenic bacteria. A mutation analysis will be performed of regions of the protein known to be involved in adhesion of L. fermentum to pig gastric mucosa. The capacity to compete with pathogenic bacteria will be determined.
Finally, other Lactobacillus strains will be screened to determine the occurrence of adhesion proteins by using antibodies against these proteins and specific DNA probes. Their adhesion properties will be tested. Training content (objective, benefit and expected impact)
The main training content is concerned with the molecular biology of colonising lactobacilli. In this way, I will become familiarized with i) procedures of gene fusion and expression of hybrid proteins in homo- and heterologous hosts, ii) mutation analysis and adherence test in Lactobacillus, being all of them complementary to those I have already carried out. So, I could continue my professional development in lactic acid bacteria, previously focused on Lactococcus.
Links with industry / industrial relevance (22)
The use of lactic acid bacteria as live delivery vectors for vaccines in mucosal immunization programmes. (Any particular information is still confidential)
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences chemical sciences organic chemistry organic acids
- natural sciences biological sciences microbiology bacteriology
- medical and health sciences basic medicine immunology immunisation
- natural sciences biological sciences biochemistry biomolecules proteins
- medical and health sciences basic medicine pharmacology and pharmacy pharmaceutical drugs vaccines
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
3700 AJ ZEIST
Netherlands
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.