Promotion and prevention of infection thread development during the colonization of pea nodule tissues by rhizobium

Objective

The infection thread is an intracellular tunnel which develops in the first steps of the nodulation process, through which the invading rhizobia infect the legume roots. Several plant components identified in the lumen of infection threads suggest a role of reactive oxygen species in regulating the rate of colonisation by Rhizobium and in controlling the success of the symbiosis. The purpose of this proposal is to study the role of pea diamine oxidase, an abundant enzyme in legume root tissues, as the potential source of hydrogen peroxide. The objectives are to demonstrate by a combination of approaches involving biochemistry and in situ localisation studies that diamine oxidase is implicated in the oxidative cross-linking of some components of the matrix of infection threads. Reporter-gene fusions and genetic modification of diamine oxidase gene expression will be achieved to investigate the importance of this gene as a regulator of nodule development.

Fields of science

• medical and health sciences › medical biotechnology › genetic engineering
• natural sciences › biological sciences › biological behavioural sciences › ethology › biological interactions
• natural sciences › biological sciences › biochemistry › biomolecules › proteins › enzymes
• agricultural sciences › agriculture, forestry, and fisheries › agriculture › grains and oilseeds › legumes

Programme(s)

• FP4-TMR - Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998

Topic(s)

• 0302 - Post-doctoral research training grants
• TL12 - Plant Sciences

Call for proposal

Data not available

Funding Scheme

RGI - Research grants (individual fellowships)

Coordinator

John Innes Centre

Address

Sainsbury Laboratory Colney Lane
NR4 7UH Norwich
United Kingdom

Participants (1)

Not available

France