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Isolation of agronomically important genes from tomato - lycopersicon esculentum - by activation tagging


Research objectives and content
The main objective of this project is to develop improved varieties of tomato regarding the following features:
1. Avoiding of side-shoot formation: Side-shoots act as sink organs, reducing the yield of the main stem, and impeding automated harvesting of the fruit. Removal of side-shoots by hand adds costs to crop production and contributes to the spread of diseases. Plants without side-shoot formation will be developed.
2. Hormone-independent growth: Plants growing in auxin- and
cytokinin-free medium will be obtained.
3. Salt tolerance: Text experiments will be performed to establish screening procedures for salt-tolerant phenotypes.
The unifying scheme in the search for these rather diverse
characters is the use of a self-stabilizing transposon tagging system based on the Ds element of Zea mays. This system will be used in different ways for the isolation of mutants:
1. Mutants with abnormalities in plant architecture: Given that seed material is available, these screenings can be started immediatly. 2. Hormone-independent growth: Seeds from transgenic plants will be germinated on selective medium and protoplasts will be isolated from cotyledons and primary leaves of resistant plants.
3. Salt tolerance: Screening conditions have to be established. Training content (objective, benefit and expected impact)
The training objective of this project is to learn the transposon tagging technique in order to implement it into citrus in the future. This technique of insertional mutagenesis would have a great impact on citrus breeding given that the low resolution of the linkage maps now available and the long vegetative phase of these species hinder the application of molecular markers.
Links with industry / industrial relevance (22)


Carl-von-linné-weg 101
50829 Köln

Participants (1)

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