Objective
This project is aimed at understanding how insulin regulates the fusion of intracellular vesicles endowed with the GLUT4-glucose transporter with the plasma membrane. In particular, we aim to identify | proteins involved in the formation of the so called 'fusogenic particle'. To this end, two main approaches will be developed. First, we will carry out in vivo functional assays investigating the effect of introducing, into living cells, inhibitory antibodies against proteins thought to play a key role in membrane fusion. Second, using two recombinant proteins (NSF and a-SNAP), which appear to be essential components of the intracellular membrane fusion apparatus, an affinity purification procedure (based on the natural binding of these proteins to their targets) will be developed to isolate proteins from immunopurified GLUT4-containing vesicles which are involved in the formation of the fusogenic machinery. Training content (objective, benefit and expected impact) The applicant will be trained in a variety of methods including generation of polyclonal and monoclonal antibodies, protein over-expression in Echerichia coli and purification of recombinant proteins, cell culture and protein purification. Other techniques include l electroporation of cells and subcellular fractionation. ; It is our belief that we, as a group, are suitably poised to address the questions raised in the attached application and that information generated from the proposed studies will contribute significantly to our conceptual understanding of how glucose transport is regulated during physiological and pathological I circumstance. Links with industry / industrial relevance (22)
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences chemical sciences electrochemistry bioelectrochemistry electroporation
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
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Coordinator
54006 Thessaloniki
Greece
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.