Objective
The Serum Response Element (SRE) is an essential regulatory element found in the promoters of many genes, including c-fos, whose transcription is rapidly and transiently induced following growth factor or mitogen stimulation. The transcription factor known as Serum Response Factor (SRF) and its accessory protein, Ternary Complex Factor (TCF), bind SRE cooperatively to form a ternary complex. Activation of SRE by TCF-linked signal pathways is relatively well understood. In addition, SRF can activate transcription independently of TCF via a novel signal transduction pathway (SRF linked signal pathway). The objective of the proposal is to characterise this pathway. This will be done using four different approaches:
- Characterisation of stimuli and inducers that activate the SRF-linked signal pathway (Investigating M1 and M2 muscarinic receptors as activators of this pathway; finding agents inducing specifically this pathway). - Several candidate regulatory factors (members of different kinase families) will be tested as activators of the SRF-linked pathway. - Identification of potential kinases involved the SRF-linked signal pathway will be tried by a series of biochemical methods.
- Cloning cDNAs that activate the SRF-linked signal pathway will be attempted using a functional cDNA library screening method. The post-doctorate will be trained in many advanced molecular biology techniques (transfection, cDNA library screening, gene expression analysis). Training will also contain all the biochemical techniques necessary for the analysis of signalling molecules, microinjection of DNAs and proteins, FACS (Fluorescence Activated Cell Sorting).
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences genetics RNA transcriptomes
- natural sciences biological sciences molecular biology
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
WC2A 3PX LONDON
United Kingdom
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