Objective
In Jurkat cells the Shc adaptor protein has been demonstrated to play a role in mediating both TCR and CD4 signals to the Ras signaling pathway whose activity is essential for NF-AT activation. The Shc protein contains two protein phosphotyrosine binding domains, the SH2 domain and PTB domain, with different specificity. Truncated forms of the Shc protein containing only the SH2 domain or the PTB domain function as dominant inhibitors of CD4 and TCR/CD4 signaling respectively. The objective of this project is to study the role of Shc in transducing TCR and CD4 signals in T-cell subsets at differented stages of T-cell development. A transgenic mouse carrying a gene coding for the inhibitory SH2 domain has been obtained. The proliferative response of peripheral T-cells to mitogens is impaired in these mice however T-cell development appears normal. This mouse will be crossed with a transgenic mouse carrying the luciferase reporter under the control of an NF-AT dependent promoter in order to facilitate the analysis of TCR and CD4 signals in the presence of the dominant negative Shc. TCR and CD4 signals will be analyzed in different subsets of T-cells from these mice. A similar line of transgenic mice will be produced with the PTB domain which should inhibit both TCR and CD4 signals. The project will ensure a thorough training in modern signal transduction research using up to date technology. In addition the close links between the molecular biology aspects of the project with those of immunology give a cross-disciplinary quality to the project. Links with industry / industrial relevance (22)
The host institue is the research center of a biotechnology company dedicated to the design and development of innovative vaccines. Basic and applied research go hand in hand to achieve these goals.
Fields of science (EuroSciVoc)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Data not available
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
53100 SIENA
Italy
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.