Objective
Research objectives and content
Antibiotic producing bacteria need an effective self-defense mechanism. In the case of antibiotics acting on elongation factor Tu, the expression of specialized resistant EF-Tus may enable the producers to survive. Firstly, the mechanism of inhibition of the antibiotic GE2270A on the peptide-chain elongation cycle and its binding to the target protein EF-Tu will be examined by analyzing the resistant EF-Tu from the GE2270A-producing Planobispora rosea and mutated EF-Tus from E. coli. The results will be compared to those described for kirromycin and pulvomycin. Secondly, OtrA, the EF-G-like oxytetracyclineresistance protein of Streptomyces rimosus, will be investigated by in vitro studies in order to elucidate its functioning in ribosome reactivation. Mutant OtrA that binds XTP instead of GTP will give a clue about the individual contributions of OtrA and EF-G/EF-Tu. Altogether, the results will not only contribute to the understanding of self-defense mechanisms in antibiotic producing organisms, but also to an expansion of current views on the fundamental process of protein biosynthesis.
Training content (objective, benefit and expected impact)
The training objective of the project is to gain experiences in in vitro and in vivo translation systems, in the isolation and functional characterization of proteins and in the analysis of antibiotic inhibition mechanisms. Besides learning technical skills, I also expect to benefit for my scientific experience from the excellent research climate at the Leiden University and the group of Dr. Kraal, in particular. Furthermore, via the active participation of the group in the EU Network ERBCHRXCT 940510 I will have favourable contacts with other member groups working on translation factors with complementary approaches such as X-ray diffraction. Links with industry / industrial relevance (22)
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences microbiology bacteriology
- natural sciences biological sciences biochemistry biomolecules proteins
- medical and health sciences basic medicine pharmacology and pharmacy pharmaceutical drugs antibiotics
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
2300 RA Leiden
Netherlands
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.