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Elucidation of the regulatory cascade controlling the expression of hrp genes involved in plant pathogenesis of pseudomonas solanacearum

Objective



Bacterial wilt caused by Pseudomonas solanacearum is one of the major bacterial plant disease world wide. In P. l solanacearum strain GMI1000 the pathogenecity determinants (hrp genes) are organized in seven transcription units. Data from the partial sequence of unit 5 and 6 have revealed the presence of two ORFs whose predicted products are homologuos to response regulators belonging to the two components regulatory systems. The present project aims at the identification of hrp genes present in transcription units 5 and 6 of the hrp cluster of P. | solanacearum, and the eludation of the role of these genes in the regulation cascade governing pathogenecity and the | interactions with other hrp genes and some plant factors.
The work programme will include: i) The delimitation of the right flanking region of the hrp gene cluster by mutagenesis | of the region spanning the putative transcription units 5 and 6. ii) Sequencing of a 4 kb DNA region downstream to the ORFs | previously identified. iii) The analysis of the genetic organization and regulation of transcription of these regions and iv) The | phenotypic characterization of mutations in each individual gene to tentatively define the functional role of each gene product | and the regulatory pathway which controls the pathogenicity in P. solanacearum. The conclusions obtained from this work will significantly enhance the understanding of the molecular determinants of pathogenecity of bacterial towards plants and may enable us to design novel environmentally-friendly crop protection strategies based on genetically engineered plants and microorganism (GMOs).
Links with industry / industrial relevance (22)
The design of novel environmentally-friendly crop protection strategies based on genetically engineered plants and microorganism (GMOs) is presently investigated by a crop protection company and has been supported by a BRIDGE contract associating this company with the proposed host laboratory.

Funding Scheme

RGI - Research grants (individual fellowships)

Coordinator

INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE
Address
Chemin De Borde-rouge
31326 Auzeville - Castanet-tolosan
France

Participants (1)

Not available
Spain