Objective
Research objectives and content
Recently, vaccinia virus has been shown to move within the host cell and from cell to cell using actin polymerization as a driving force (Cudmore et al., 1995, Nature 378, 636-638; Cudmore et al., 1996, J. Cell Sci., In press). This project aims to identify the viral protein that initiates the cascade of events that lead to the polymerization of the actin tail that propels the virus particle as well as the host factors involved in this process. Actin tails are only induced by the IEV (intracellular enveloped virus) form of vaccinia, whose outer membrane surface is derived from the trans Golgi network (TGN). Through a combined screen and biochemical approach, we will identify the viral protein present at the IEV surface that is responsible for initiating the cascade of events that lead to actin tail formation and ultimately movement. In addition, we will develop in vitro assays using cell extracts from a variety of sources to facilitate identification and study of both viral and host proteins involved in the actin based motility of vaccinia virus.
training content (objective, benefit and expected impact)
The main objective is the understanding the spread of vaccinia as a model to understand the spread of viruses. Taking advantage of my yeast actin cytoskeleton background, the post-doctoral project described here will permit my entrance into the domain of mammalian cytoskeleton and viral pathology. I will benefit from training in the handling of viruses and tissue culture as well as in in vitro assays to study motile processes. EMBL will also be the perfect place to learn new techniques such as electronmicroscopy. Identification of viral and host factors involved in the motile process will be an important first step in the molecular characterization of the role of the cytoskeleton in viral infection. Links with industry / industrial relevance (22)
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences health sciences infectious diseases RNA viruses
- natural sciences biological sciences microbiology virology
- natural sciences biological sciences biochemistry biomolecules proteins
- medical and health sciences basic medicine pathology
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
69117 Heidelberg
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.