Objective
Research objectives and content
Sporulation in Bacillus involves an asymmetric cell division. The mother cell subsequently engulfs the smaller forespore and they collaborate in developing the heat-resistant spore coat.
Two key proteins which direct late events in the mother cell during sporulation are GerE and SpoVM. GerE is a 74 residue DNA-binding protein which acts as an activator and repressor of transcription. SpoVM is required for cortex and coat formation and plays a morphogenic role. I proposed to isolate the gerE gene from B. stearothermophilis and subclone it for overexpression. The recombinant protein will be purified, characterised in DNA binding assays and used in crystallisation experiments. The spoVM protein has been chemically synthesised and crystallisation trials can begin immediately.
Training content (objective, benefit and expected impact)
My background is in protein crystallography and it is my intention to continue working in structural biology. It is important for me to acquire recombinant DNA techniques for the overexpression and mutagenesis of genes encoding proteins of interest. The York laboratory combines expertise and facilities in molecular biology, protein biochemistry and crystallography. This proposal gives me training opportunities in recombinant DNA techniques. These, together with my present experience, will allow me to become an effective independent scientist able to identify and solve problems.
Links with industry / industrial relevance (22)
Not applicable
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics DNA
- natural sciences earth and related environmental sciences geology mineralogy crystallography
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences molecular biology structural biology
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
YO1 5DD York - Heslington
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.