Objective
Research objectives and content
The objective of the project is to devise new optical spectroscopic methods for characterising the molecular mechanism of the interaction of calmodulin with its target sequences and for studying the conformation of calmodulin when bound to different target enzymes. Calmodulin activates many enzymes in a calcium dependent process, and is the link: between changes in intracellular calcium concentration and the activation of specific enzymic processes. The project aims to study and extend existing biophysical methods (which have used complexes of calmodulin with peptides of defined sequence) to study calmodulin-enzyme interactions. This will involve the thermodynamic characterisation of calmodulin-peptide complex formation, design and expression of calmodulin mutants, studies of their conformation and stability, preparation of suitable fluorescent and phosphorescent calmodulin derivatives, and studies of the complexes with peptides and enzymes involving potentially different modes of calmodulin binding.
Training content (objective, benefit and expected impact)
The project will allow the candidate to carry out a PhD programme and to widen the competence acquired with the Italian degree. In particular, the candidate will extend her knowledge of problems like protein structure and stability, and the relationship between protein functions and conformations. She will also gain wider laboratory experience in biophysical and spectroscopic methods, and she will learn useful biochemical and molecular biology techniques important for further research in biophysical studies on proteins.
Links with industry / industrial relevance (22)
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences chemical sciences inorganic chemistry alkaline earth metals
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
- natural sciences biological sciences molecular biology
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Data not available
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
NW7 1AA London
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.