Objective
Research objectives and content
To study nuclear RNA export, an in vitro approach is proposed based on the assembly of synthetic nuclei in Xenopus egg extracts. These nuclei are functional for protein import, they transcribe the intranuclear U1 snRNA reporter gene and they actively export the RNA. The aim is to elucidate the mechanism by which the cap binding complex (CBC) mediates U snRNA export and to identify putative other factors involved. Affinity purified antibodies raised against CBC will be used in this system to inhibit export directly and to immunodeplete extracts for CBC. Mutant versions of recombinant CBC will be added to the depleted extracts in an attempt to determine the region of CBC that is required for export. Additionally, extracts will be biochemically fractionated in order to identify other factors involved in snRNA export. A similar approach will be used to study nuclear export of different classes of RNA.
Training content (objective, benefit and expected impact)
In Dr. Mattaj's lab there is a lot of experience and knowledge about nucleocytoplasmic transport and many recombinant (mutant) transport factors are available. With the described project I could profit from this and learn new techniques (e.g. expression/purification of recombinant proteins, chromatography) and extend my knowledge in the field.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences genetics RNA
You need to log in or register to use this function
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Data not available
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
HEIDELBERG
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.