Objective
Research objectives and content
We propose to characterise the structure and the function of two VDAC (voltage-dependent anion selective channel) isoforms from the outer membrane of plant mitochondria: Plant seeds are used because it allows large scale purification of two VDAC isoforms. In order to establish a structure-function relationship for these channel we will combine two methodological approaches.
Secondary structure and topology
Attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) will be used to study the secondary structure and the orientation of the entire VDAC molecules in the membrane as well as peptide fragments (obtained after proteolysis). Experiments will be made to follow the structural changes associated to the voltage dependence of the channel properties. ATR-FTIR offers an unique possibility to provide structural information about secondary structure and secondary) structure changes in a lipid environment.
Functional characterisation
We will characterise electropysiological differences of purified VDAC isoforms at a single channel level (conductance. selectivity. probability of openings etc.) and study how phosphorylation regulates the channel properties.
Training content (objective, benefit and expected impact)
The implementation of this project will provide me a high quality training in the several techniques whose application can be extended to any other membrane channel protein. This will give me the opportunity to develop and carry out new research projects in other laboratories within the European Union.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences mathematics pure mathematics topology
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences biochemistry biomolecules lipids
- natural sciences physical sciences optics spectroscopy absorption spectroscopy
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Programme(s)
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
1050 BRUXELLES
Belgium
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.