Objective Research objectives and content In my proposed project I plan to use a novel approach developed in Bernhard Herrmann's laboratory to isolate and describe expression patterns of a large majority of genes active in midgestation mouse embryos. This approach is based on using cDNA riboprobes derived from characterized stage specific cDNA libraries for multiple in situ hybridizations of whole-mount embryos. Using a large scale application of this technique I will generate expression patterns of up to two thousand different genes in 9.5 - 12.5 day mouse embryos. From the projected number of expression patterns I will choose to further characterize genes likely to interact downstream of Brachyury (T) in mesoderm and notochord by "knock out" mutagenesis and functional analysis. In summary this project should result in an expression catalogue of 2000 different genes of 9.5 -12.5 day embryos and should provide new insights towards understanding of mesoderm formation but also in a multitude of new molecular markers of mouse embryo. Training content (objective, benefit and expected impact) Personally this training grant would facilitate to do this project and make it possible for me to learn many new techniques and establish myself in the fields of developmental biology. Links with industry / industrial relevance (22) In a pilot project of the in situ screen in Bernhard Herrmann's laboratory about 10 to 15 new genes have been identified specifically expressed in blood cells and/or cells of the blood endothel. It is expected that a similar number of new genes specific for these tissues will be discovered as a sideproduct in my proposed project. These genes are clearly of industrial relevance in sight of medical research of blood endothel and hematopoiesis. Fields of science medical and health sciencesbasic medicineanatomy and morphologynatural sciencesbiological sciencesdevelopmental biologynatural sciencesmathematicspure mathematicsmathematical analysisfunctional analysismedical and health sciencesclinical medicineembryology Programme(s) FP4-TMR - Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998 Topic(s) 0302 - Post-doctoral research training grants TL06 - Developmental Biology Call for proposal Data not available Funding Scheme RGI - Research grants (individual fellowships) Coordinator Max-Planck-Gesellschaft zur Förderungder Wissenschaften e.V. EU contribution No data Address 51,Stübeweg 79108 Freiburg Germany See on map Total cost No data Participants (1) Sort alphabetically Sort by EU Contribution Expand all Collapse all Not available Austria EU contribution No data Address See on map Total cost No data
