Objective
Research objectives and content
In my proposed project I plan to use a novel approach developed in Bernhard Herrmann's laboratory to isolate and describe expression patterns of a large majority of genes active in midgestation mouse embryos. This approach is based on using cDNA riboprobes derived from characterized stage specific cDNA libraries for multiple in situ hybridizations of whole-mount embryos. Using a large scale application of this technique I will generate expression patterns of up to two thousand different genes in 9.5 - 12.5 day mouse embryos. From the projected number of expression patterns I will choose to further characterize genes likely to interact downstream of Brachyury (T) in mesoderm and notochord by "knock out" mutagenesis and functional analysis. In summary this project should result in an expression catalogue of 2000 different genes of 9.5 -12.5 day embryos and should provide new insights towards understanding of mesoderm formation but also in a multitude of new molecular markers of mouse embryo.
Training content (objective, benefit and expected impact)
Personally this training grant would facilitate to do this project and make it possible for me to learn many new techniques and establish myself in the fields of developmental biology.
Links with industry / industrial relevance (22)
In a pilot project of the in situ screen in Bernhard Herrmann's laboratory about 10 to 15 new genes have been identified specifically expressed in blood cells and/or cells of the blood endothel. It is expected that a similar number of new genes specific for these tissues will be discovered as a sideproduct in my proposed project. These genes are clearly of industrial relevance in sight of medical research of blood endothel and hematopoiesis.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences basic medicine anatomy and morphology
- natural sciences biological sciences developmental biology
- natural sciences mathematics pure mathematics mathematical analysis functional analysis
- medical and health sciences clinical medicine embryology
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
79108 Freiburg
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.