Research objectives and content
In my proposed project I plan to use a novel approach developed in Bernhard Herrmann's laboratory to isolate and describe expression patterns of a large majority of genes active in midgestation mouse embryos. This approach is based on using cDNA riboprobes derived from characterized stage specific cDNA libraries for multiple in situ hybridizations of whole-mount embryos. Using a large scale application of this technique I will generate expression patterns of up to two thousand different genes in 9.5 - 12.5 day mouse embryos. From the projected number of expression patterns I will choose to further characterize genes likely to interact downstream of Brachyury (T) in mesoderm and notochord by "knock out" mutagenesis and functional analysis. In summary this project should result in an expression catalogue of 2000 different genes of 9.5 -12.5 day embryos and should provide new insights towards understanding of mesoderm formation but also in a multitude of new molecular markers of mouse embryo.
Training content (objective, benefit and expected impact)
Personally this training grant would facilitate to do this project and make it possible for me to learn many new techniques and establish myself in the fields of developmental biology.
Links with industry / industrial relevance (22)
In a pilot project of the in situ screen in Bernhard Herrmann's laboratory about 10 to 15 new genes have been identified specifically expressed in blood cells and/or cells of the blood endothel. It is expected that a similar number of new genes specific for these tissues will be discovered as a sideproduct in my proposed project. These genes are clearly of industrial relevance in sight of medical research of blood endothel and hematopoiesis.