Objective
Research objectives and content
Escherichia coli is an important pathogen causing both intestinal and extraintestinal infections. In diseases caused by Escherichia coli, attachment to host cell tissue is a key step in establishing and maintaining infections. Type 1 fimbriae are the most ubiquitous adhesin found amongst Escherichia coli isolates. Expression of type 1 fimbriae is phase-variable and controlled by a genetic switch that can be turned off rapidly under certain conditions. Nothing is known about the loss of fimbriae from the bacterial surface once transcription has ceased. Given the highly immunogenic nature of fimbriae, the ability to become afimbriate quickly may be of importance for the survival of the bacteria during infection. This project aims to investigate the loss of fimbriae from Escherichia coli once transcription has been switched off. This process will be followed by using a variety of methods including Western blot analysis for the main fimbrial subunit protein and single cell fluorescence microscopy.
Training content (objective, benefit and expected impact)
Training will be focused on the proposed protein analysis and fluorescence microscopy, in particular the use of specialized software for analysis of the fluorescence images. It is anticipated that the research will lead to an application for EU funding as part of a collaboration between the two institutes.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences computer and information sciences software
- natural sciences biological sciences microbiology bacteriology
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences physical sciences optics microscopy
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
NE2 4HH Newcastle - Upon Tyne
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.