Objective
Research objectives and content
My research project consists of two independent studies that will be pursued in parallel. The results of the experiments will determine which project will receive emphasis. The objectives of these projects are outlined below:
Analysis of Krox-20 regulation in rhombomere 3 (r3) and rhombomere 5 (r5) DNA centered around the Krox-20 gene has been cloned in three cosmid vectors. I will subclone several fragments into a reporter construct which contains the lacZ gene driven by the B-globin promoter. This promoter lacks specific expression in the developing hindbrain but is very sensitive to enhancer activity. Transgenic mice will be generated and examined for lacZ expression in r3 and r5. Enhancer elements will be localized by deletion mapping and specific binding sites will be identified by homology searches and gel retardation analysis using extracts of mouse or chick embryos. Phenotypic analysis of Krox-20 and Krox-24 double mutants
Double mutant mice defective in both functional Krox-20 and Krox-24 will be generated by crossing mice heterozygous for each gene followed by crossing the double heterozygotes. A detailed phenotypic analysis involving morphologic, histologic well as molecular analyses will be performed to identify phenotypic abnormalities that are unique to the double mutants and may reflect areas of functional redundancy or rescue between these genes. Training content (objective, benefit and expected impact)
We expect that the work on the promoter of Krox-20 will provide a first insight at the factors governing early patterning in the hindbrain. The double mutant studies will complement previous studies on Krox-20 and Krox-24 mutant mice and increase understanding of the role of these transcription factors in development. These studies involve a wide range of techniques that are critical for research in understanding developmental processes. It is my goal to acquire a strong basis of knowledge in the area of vertebrate development. This training in Dr. Charnay's laboratory will form a foundation on which to build my care in this field.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics DNA
- medical and health sciences clinical medicine embryology
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
75230 PARIS
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.