Objective
Research objectives and content
This proposal concerns the regulation of mitosis. The activity of the mitotic kinase cdc2 is tightly controlled by different regulatory proteins. An important aspect is where these factors are in the cell. Cyclins, cdks and cdc25s all comprise multigene families where one major difference between the family members is their subcellular localization. We are using Green Fluorescent Protein (GFP) as a marker to follow the localization of cell cycle proteins in living cells. We have made chimeras of GFP and different cell cycle regulators. Single cells expressing the chimeras are being followed through the cell cycle by time-lapse video-microscopy. Using this technique, I wish to investigate further the activation of cdc2 by cyclin B 1 binding and cdc25 phophatase activity. This also involves the use of mutants that are deficient in localization and/or function.
Training content (objective, benefit and expected impact)
In Jonathon Pines' laboratory, advanced new technology is used to study protein dynamics in living cells. I would like to become familiar with these techniques i.e. fluorescence-video microscopy, confocal microscopy and image processing. I will also use microinjection, in vitro mutagenesis and antisense technology. These methods, together with the use of GFP as fluorescent marker has numerous applications in cell biology and will potentially be very useful in my future career.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences cell biology
- natural sciences physical sciences optics microscopy confocal microscopy
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
CB2 1QR Cambridge
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.