Objective
Research objectives and content
Initiation of DNA replication is a key step in the regulation of the cell division cycle. The commitment to enter S-phase occurs late in G1 phase at the so-called restriction point. Both events are control in part by Gl cyclins and their cyclin- dependent kinases. Recently a novel cell free system has been developed in the group of Prof. Ron Laskey in which semi-conservative initiation of DNA replication was shown in G, phase nuclei from HeLa cells when incubated with cytosolic and nuclear factors from S-phase HeLa cells. Exploiting this novel human system this study will investigate coupling of signal transduction pathways to the initiation of DNA replication.
Training content (objective, benefit and expected impact)
Knowing that cyclin A/Cdk2 and cyclin E/Cdk2 stimulate replication synergistically, I will investigate the function of cyclin D1/Cdk4 complex and its relation to the other G1 cyclin complexes by adding the recombinant purified proteins. Furthermore novel factors such as a already characterised inhibitory activity in G1 nuclear extracts will be isolated by biochemical fractionation. Finally a sequence of action of regulatory initiation proteins might be defined. Understanding coupling of DNA replication to the cell cycle will have impact on the research of the genetic changes that lead to cancer.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences biochemistry biomolecules proteins
- medical and health sciences clinical medicine oncology
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
CB2 1QR Cambridge
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.