Objective
Research objectives and content
Based on a new structural NMR parameter that complements the Nuclear Overhauser Effect and scalar coupling constants, angles between bonding vectors in proteins and oligonucleotides will be measured and the impact on structure elucidation and the dynamics of the biomacromolecules will be explored.
The new method relies on relaxation via cross-correlated dipolar couplings between the four nuclei that form the two internuclear vectors under consideration. The corresponding relaxation rates can be derived from the relative intensities of the components of a double quantum multiplet. These rates depend on the angle between the bond vectors, which can therefore be measured directly, without any need for empirical calibration.
New insights into slow segmental motion of the structure of
biomacromolecules, that is related to their function, is expected from these measurements The methodology will be applied to the
thrombin-inhibitor rhodniin, the calcium binding protein calmodulin as well as to a hairpin loop from the plasmid replication control system of E. coli.
Training content (objective, benefit and expected impact)
A new method to measure bond angles, which does not rely on coupling constants and empirical calibrations, will be further developed to obtain insight in the structure and dynamics of biomacromolecules. This parameter is expected to be of great help in obtaining a better accuracy of structures of pharmaceutically relevant molecules in solution. The project requires a sound theoretical and experimental training, from which I will greatly benefit, improving my skills and promoting my education in the NMR field My scientific experience in the development of NMR methods for protein structure determination will contribute to the project.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- engineering and technology electrical engineering, electronic engineering, information engineering electronic engineering control systems
- natural sciences chemical sciences inorganic chemistry alkaline earth metals
- natural sciences biological sciences biochemistry biomolecules proteins
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
60439 FRANKFURT AM-MAIN
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.