Objective
Research objectives and content
The major focus of this proposal is to study the mechanisms of protein transport through the Golgi compartments in yeast. Based on previous studies, transport through the Golgi is thought to be SNARE-mediated. Two v-SNAREs (Sft1p and p28) are known in the yeast Golgi. Moreover, two candidates t-SNAREs (tsx and tsz) have been identified by homology searches and are located in the Trans Golgi Network. We will investigate the role of these proteins in the intra-Golgi anterograde transport using genetic approach and various in vivo functional assays. The oartners of tsx/tsz in SNARE complexes (Sft1p, p28 or others) will be identified. A related study will consist in the characterization of the nature of connections between Golgi compartments. We will try to observe budding and fusion events directly in vivi using the Green Fluorescent Protein technology. Moreover, a cell free functional assay for intra-Golgi transport will be developped. These two methods will be very helpful to determine whether intra-Golgi anterograde traffic is vesicle-mediated and to analyse in detail the biochemical requirements of these transport processes.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
CB2 2QH Cambridge
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.