Objective
Research objectives and content There is an inverse correlation between incidence of atherosclerosis and the HDL levels. This protective activity of HDL is generally attributed to its role in the reverse, cholesterol transport, mechanism by which peripheral cells transport cholesterol excess to the liver to elimination through the bile. And so, reverse cholesterol transport seem to be a good potential therapeutic target for treating atherosclerosis. In the project, we want to enhance reverse cholesterol transport using somatic gene transfer by overexpression of apoA-I (the major protein of the HDL) in the liver and validate reverse cholesterol transport as a therapeutic target in gene-therapy for atherosclerosis. This will be made after construction of plasmid and adenoviral vectors with gene insert of apoA-I, and transfection into cultured cells or atherosclerotic animal models. We will follow apoA-I concentration and expression level of apoA-I gene to verify the overexpression expected. Then, we will look for the impact of this overexpression at the metabolic and physiological levels. Transfected cells and animals will be used for cellular cholesterol efflux measurements. The lipoprotein profile, LCAT activity (and biliary secretion of cholesterol for the animals) will be studied on these models, accompanied by a physiological study of the atherosclerosis development on the animals. Training content (objective, benefit and expected impact) This project will allow us to validate reverse cholesterol transport as therapeutic target in gene-therapy for atherosclerosis.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences medical biotechnology genetic engineering gene therapy
- medical and health sciences clinical medicine cardiology cardiovascular diseases arteriosclerosis
- natural sciences biological sciences biochemistry biomolecules proteins
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
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Coordinator
54006 Thessaloniki
Greece
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.