Objective
Research objectives and content
The objective is to define the function of a novel human DNA mismatch recognition activity. The host laboratory has identified a heterodimeric (subunits of approximately 140kDa and llOkDa) human mismatch binding complex which appears not to be required for postreplicative mismatch repair. The llOkDa component is a member of the SMC protein family. This family also includes the S. pombe RAD 18 and E. coli RecN gene products which are involved in recombinational DNA repair and the rejoining of double-strand DNA breaks. Recently, a bovine SMC homolog has been shown to be associated with a complex that promotes DNA recombination-like activities in vitro. The size and properties of this activity resemble those of the human mismatch recognition complex.
Peptide sequence obtained by mass specrometry in the host institution identified expressed sequence tags (EST) for both proteins. The clones have been obtained and will be used to isolate full length cDNAs. In vitro protein expression systems, including the yeast two hybrid system, will be used to investigate the interactions between the two component proteins of the complex and with other cellular proteins. This approach will help define the role of this mismatch recognition complex in recombination and/or DNA repair.
Training content (objective, benefit and expected impact)
The host laboratory has extensive experience in biochemical approaches to DNA repair, and in particular DNA mismatch repair. The candidate will obtain biochemical expertise in protein characterization and will learn how to carry out biochemical assays for DNA mismatch repair related functions. The candidate's own experience of the yeast two-hybrid system will be an essential asset in investigating the protein:protein interactions of the new mismatch recognition activity.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences biochemistry biomolecules proteins
You need to log in or register to use this function
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Data not available
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
EN6 3LD Potters Bar
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.