Research objectives and content
The in vivo compaction of eukaryotic DNA with histones is a formidable barrier to chromatin transcription. Increased histone acetylation is linked to chromatin-associated gene activation, but the molecular mechanism underlying this relationship is still largely unknown. The important discovery that CBP protein has histone acetyltransferase activity which contributes to acetylation, offers new approaches to this question. CBP acts as a co-activator of the E2F1 transcription factor. My project aims at investigating the relevance of the CBP acetylase activity for E2F1-mediated transcriptional activation as well as the mechanism involved in this process. This study should help to understand the molecular basis of how histone acetylation is linked to transcription and should factor significantly into understanding of gene expression from natural chromatin templates.
Training content (objective. benefit and expected impact)
A main objective of the requested training is to greatly improve my scientific knowledge and technical skill in my own research field. Concretely, I expect to learn a novel technique developed and currently only mastered by the host laboratory. I also expect to benefit from the high-profile research of the host group which contributes regularly to important new findings and is at the forefront of my own scientific area.