Objective
The primary event in gene transcription is the recognition of promoter sequences by transcription factors. One particularly interesting case is the binding of yeast transcription factor TFIIIC (z or tau) to tRNA genes. The elucidation of the molecular structure of X and the identification of the polypeptides that contact DNA are prerequisites to our understanding of the mechanism of tRNA gene activation. We have made some observations that hint at an important role for the 91 kDa subunit of TFIIIC (X91 ) in class III gene expression: It is the only TFIIIC subunit that can bind a nucleic acid on its own and there is a possible link between the transcription and RNA maturation processes (I have observed a strong RNA maturation defect in a z138 mutant that was suppressed in a n138+T91 double mutant). My research project aims at strengthening these observations and at establishing the role of T91 in TFIIIC. I shall first confirm that T91 iS part of TauB, the TFIIIC domain that binds to the B block of tRNA genes and has a predominant role in stabilizing T-DNA complex, then focus on the interactions engaged by T91 at the DNA, RNA or protein level.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules nucleic acids
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences genetics RNA
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
91191 Gif-sur-Yvette
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.