Research objectives and content
We plan to do genetic studies in natural populations of Antirrhinum. After collecting a large sample of plants from two different geographical populations, we plan to establish genetic distances between populations, using allozyme genetic markers. These results will also be helpful to verify the breeding systems of these populations which can be estimated using genotype frequencies at several allozyme loci. This results will be confirmed with controled plant crosses. Sequence data on the self-incompatibility S-locus, will be used to estimate diversity levels at this locus, and will provide us with tools to test theoretical models on the relationship between levels of genetic diversity and levels of inbreeding. It will also be used to identify regions of the S-locus that are functionally important. Diversity in inbreeding and outcrossing populations at S-related loci will also be estimate, using the same technical approach.
Training content (objective, benefit and expected impact)
The molecular biology work I did previously at the Daniel Hartl laboratory (Harvard University) gave me not only the necessary technical training for this project, but also some theoretical training in population genetics and evolution. The proposed work will improve my skills in molecular biology, but also, and most importantly to explore a more theoretical evolutionary aspect, complementing my previous formation.
Links with industry / industrial relevance (22)