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Content archived on 2024-04-30

The intertwined roles of proteins in transcription and dna rapair

Objective



Research objectives and content
DNA damages in actively transcribed genes are removed much faster than in the genome overall, indicating a link between transcription and DNA repair. It also suggests that a specific repair mechanism exists for recognizing stalled RNA polymerases in the elongation stage of transcription. This project proposes to investigate transcriptional elongation and transcription-coupled repair by studying the function of two multi-subunit factors thought to play crucial roles in these processes, TFIIH and elongator. I propose to use a combination of biochemistry and genetics - such as protein purification and characterization; determination of protein functions in vitro and in vivo; gene disruption and genetic analysis; etc - with yeast as a model system.
Training content (objective, benefit and expected impact)
By doing research at Dr. Svejstrups lab at the ICRF, I will learn a large number of new techniques in all fields of molecular biology/biochemistry. The research proposed herein will bring a deeper understanding of the processes of transcription and DNA repair. Ultimately, since regulation of transcription, DNA repair and cell cycle control are coupled - enabling cells to stop transcription and cell cycle progression until the DNA damage is repaired - it should gain a better insight in cancer as well. Links with industry / industrial relevance (22)

Programme(s)

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Topic(s)

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Call for proposal

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Funding Scheme

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RGI - Research grants (individual fellowships)

Coordinator

IMPERIAL CANCER RESEARCH FUND
EU contribution
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Address
Blanche Lane - South Mimms
EN6 3LD POTTERS BAR
United Kingdom

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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

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