Objective
Research objectives and content
Lipid translocation to the outer leaflet of the plasma membrane The objective is to determine how phospholipids reach their destination within the cell. In contrast with proteins the final localisation of a phospholipid molecule depends on it's intracellular transport between organelles and it's translocation (or 'flipping') between the two leaflets of the bilayer. The first stage of the project will characterise the translocation of natural lipid by the human multidrug resistance proteins MDR1 and MDR3. Methods already developed in the host laboratory will be used to permeabilize selectively one of the two plasma membrane domains of LLC PK1 cells grown on filters. Addition of liposomes of natural radiolabelled phospholipid in the presence of the appropriate transfer protein to the non-permeabilized surface will make it possible to assay translocation across the plasma membrane to the permeabalized side. This can be achieved by either the use of an appropriate bacterial phospholipase C in a kinetic assay or by using an appropriate transfer protein and a liposome acceptor. Further experiments, in primary cells from knock out mice, will determine the role of phospholipid transfer proteins in intracellular transport in intact cells.
Training content (objective, benefit and expected impact)
The objective is to do excellent science in one of the leading laboratories in the field of phospholipid traffic in polarised cells. The proposed project provides me with training in molecular biology, cell biology and lipid biochemistry which is necessary for the long-term goal of my research, namely to contribute to the elucidation of how lipids are transported and sorted on route to the plasma membrane and the sites and regulation of synthesis of phospholipids. I am very confident that I will be able to publish in high impact journals and to establish myself as a recognised research fellow in the field.
Links with industry / industrial relevance (22)
No
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences cell biology
- natural sciences biological sciences biochemistry biomolecules lipids
- medical and health sciences basic medicine pharmacology and pharmacy drug resistance multidrug resistance
- natural sciences biological sciences molecular biology
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
1105 AZ Amsterdam
Netherlands
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.