Objective Research objectives and content This project aims to analyze the spatial and replication aspects of the regulation of beta globin gene expression and will be focused on two questions: the restoration of transcription after cell division and the spatial distribution of the globin genes in the nucleus of transgenic mice. In particular existing transgenic mice will be used to search for a mitosis 'memory protein' and the spatial distribution of the locus in mice that either contain a normal globin locus or one that is subjected to position effects. In addition a novel beta globin locus will be constructed in transgenic mice to address contacts between distant regulatory regions and the genes. At present almost nothing is known about these parameters in vertebrate cells in vivo. The beta globin locus has been studied in great detail and the existing resources in beta globin transgenic mice and novel FISH technology offer an excellent opportunity to start addressing the spatial and temporal aspect of gene regulation in mammals. Training content (objective, benefit and expected impact) I will obtain training in homologous recombination cloning, genetic manipulation of mice, DNA and PNA based FISH technology, protein based fluorescence technology and 3D analysis technology including confocal microscopy. Links with industry / industrial relevance (22) None are foreseen at the moment. Fields of science natural sciencesbiological sciencesgeneticsDNAmedical and health sciencesmedical biotechnologygenetic engineeringnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural sciencesphysical sciencesopticsmicroscopyconfocal microscopynatural sciencesbiological scienceszoologymammalogy Programme(s) FP4-TMR - Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998 Topic(s) 0302 - Post-doctoral research training grants TL06 - Developmental Biology Call for proposal Data not available Funding Scheme RGI - Research grants (individual fellowships) Coordinator Erasmus Universiteit Rotterdam Address 50,dr. molewaterplein 3000 DR Rotterdam Netherlands See on map EU contribution € 0,00 Participants (1) Sort alphabetically Sort by EU Contribution Expand all Collapse all Not available Italy EU contribution € 0,00 Address See on map
