Objective
Research objectives and content Pax-6 gene is responsible for the small eye (Sey) mouse mutation. Previous studies suggest cortex malformations in the developing brain of Sey/Sey mice. The aim of the project is to investigate the mechanism which is disrupted during cortical neurogenesis in the Sey mouse. I will try to study in vitro the migration of neural cells in cortical development by developing an organ culture system using brain slices from mouse embryos. With this approach, the analysis of neurogenesis may be extended to stages several days after birth and we will be able to follow the fate of neurons in Sey mice. The process of cell migration can be followed directly over time by labelling cells using a marker gene which when expressed is non toxic and detectable in living cells. I will use two reporter genes, the gene encoding the jellyfish green fluorescent protein (GFP) and the bacterial LacZ gene. I will use lipophilic dyes, viral and retroviral vectors to label cells. The premigratory cells of the ventricular zone are a population susceptible to retroviral infection and I will use retroviral vectors to infect these proliferating cells. In addition adenovirus vectors will be use to infect not only mitotic but also postmitotic cells, labelling cells in any part of the brain. Training content (objective, benefit and expected impact)
This methodology will permit examination of the migration pattern of neuronal cells of Sey/Sey as compared to wildtype cells. The proposal try to answer some fundamental questions about the migratory route of affected neurons and the role that Pax-6 has in this deffects. In addition the proposed proyect will give me the opportunity to adquired experience in many embryological techniques required in the analysis of development and background in this area.
Links with industry / industrial relevance (22)
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences genetics mutation
- medical and health sciences clinical medicine embryology
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Programme(s)
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
EH4 2XU EDINBURGH
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.