Objective
Research objectives and content ATP is the universal energy currency of life. All living cells need ATP to maintain themselves, to move and to reproduce. Understanding the molecular mechanism by which ATP is synthesised is a major goal of modern biochemistry. ATP is synthesised by ATP-synthase which is a large, lollipop-shaped molecule found in all cells. Although it is known that the soluble part, F1 ATPase, is powered by electrochernical gradients in the cell, the precise way in which this molecular machine is able to synthesise ATP is not known. The recently solved crystal structure of the native enzyme suggests that it may act like a turbine, with the rotation (and attendant ATP-synthesis) being driven by changes in the structure of the molecule itself. My goal is to extend the existing structure to higher resolution and determine the high resolution crystal structures of conformational states of the F1-ATPase that are relevant to catalysis. This will be archived by co-crystallising the nucleotide free enzyme with nucleotides, nucleotide analogs and inhibitors which arrest the enzyme in a particular conformation. To obtain highest resolution possible, the data will be collected at liquid nitrogen temperature at high energy synchrotron sources. Training content (objective, benefit and expected impact) The objective of this project is to become more familiar with the methods involved in determination and refinement of macromolecular crystal structures. The benefit would be to be able to conduct independent research after finishing this training period. Structural biology is a modern and greatly expanding field that has a great demand for qualified scientist familiar with the latest technology. Links with industry / industrial relevance (22)
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- engineering and technology materials engineering crystals
- natural sciences biological sciences genetics nucleotides
- natural sciences chemical sciences catalysis
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
- natural sciences biological sciences molecular biology structural biology
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
CB2 2QH Cambridge
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.