Objective
Research objectives and content
Recently, Paul Brey' s group at the Pasteur Institute in Paris sequenced the promoter region of Prophenoloxidase (proPO) gene isolated from the dipteran Anopheles gambiae. They found some interesting regions highly homologous to several responsive elements like ecdysteroid hormone responsive elements, GATA elements etc. At the moment, A. gambiae has the only proPO gene completely sequenced and this study will be important in further studies to render A. gambiae inefficient to harbor the malaria parasite. In this project we want to investigate the functionality of that region of the proPO gene promoter. Furthermore, we want to verify whether this putative regulatory region of the promoter can affect proPO gene expression. Our intention is to use DNaseI footprinting experiments combined with gel shift assay in two inducible systems (the larval haemolymph and a tumorous Drosophila blood cell line (mbn-2)), to investigate the functionality of the regulatory region of the proPO gene promoter. Further, we will use the transfection assay of Drosophila tumorous blood cell to verify a possible involvement of the regulatory region of the promoter in the proPO gene expression. This project will address the very important question as to whether the possible presence of some factor in the haemolymph of A. gambiae affects the expression of the proPO gene in this environmentally important insect: a known vector of human malaria.
Training content (objective, benefit and expected impact)
This experience will be of great importance for the high quality of research and in the training provided by the host Institute. Moreover, the applicant will learn many techniques of biochemistry and molecular biology which he will in order to complete his scientific training in insect immunity.
Links with industry / industrial relevance (22)
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry
- medical and health sciences health sciences infectious diseases malaria
- natural sciences mathematics pure mathematics mathematical analysis functional analysis
- natural sciences biological sciences zoology entomology
- natural sciences biological sciences molecular biology
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
75724 Paris
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.