Objective
Research objectives and content
We propose to develop a new tool for non destructive gene trapping by transposon tagging, in order to isolate genes induced during fruit development in tomato. Transposon tagging allows the identification of genes on the basis of mutant phenotypes resulting from the insertion of a known transposable element carrying a reporter gene. The
Beta-glucuronidase gene is the most widely used reporter gene in plants, but detection of its expression requires staining which is destructive for the plant under study. We propose to use the maize gene, Lc, as a non destructive visual reporter gene. This gene is a transcriptional factor which activates the anthocyanin pathway. It was shown to be expressed in tomato and to cause purple pigmentation in a normally non purple tissue. A new tomato model will be used for this purpose. It consists of the miniature Microtom tomato cultivar, which can be growth at high density, and has a short life cycle. A Ds-Lc-trap within Microtom offers an unique system to isolate genes involved in fruit development and to characterize their spatial and temporal pattern of expression. Training content (objective, benefit and expected impact)
The training content of this research will be to learn T-DNA construction, plant transformation, transposon tagging and gene expression analysis. As very little is known on the genes involved in fruit development in tomato, the most important crop for the vegetable market, impact of this project will be both scientifically and technologically important.
Links with industry / industrial relevance (22)
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- engineering and technology materials engineering amorphous solids amorphous semiconductors
- agricultural sciences agriculture, forestry, and fisheries agriculture horticulture fruit growing
- natural sciences biological sciences genetics RNA transcriptomes
- agricultural sciences agriculture, forestry, and fisheries agriculture horticulture vegetable growing
- agricultural sciences agriculture, forestry, and fisheries agriculture grains and oilseeds cereals
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
76100 REHOVOT
Israel
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.