Objective
Research objectives and content
The DNA sequence of the Arabidopsis genome is being determined. The function of genes identified by sequencing will be a major focus of biological research. I will (with others) develop a 'mutation machine' in Arabidopsis which will facilitate the isolation of insertion mutations in any known DNA sequence. Analysis of mutant phenotypes will give information on gene function. The approach involves saturation insertion mutagenesis; identification of mutations in specific genes can then be achieved with a PCR screen. We will use the En(Spm)/l(dSpm) transposon system which is active in Arabidopsis. By selecting directly for transposed dSpm elements, we aim to produce at least 57,600 transposants. With this level of saturation, it should be possible to identify an insertion in any Arabidospsis gene (average size of 3.2 kb) with a probability of 82%. The mutation machine is being developed in the Jones lab to test whether certain genes whose induction or activation has been correlated with defense response really play an indispensable role in resistance. In particular, to test the mutation machine, I will target the following genes: (1) NADPH oxidase genes homologues from mammalian NADPH oxidases (2) calcium-dependent protein kinases (3) glucanases and chitinases. Training content (objective, benefit and expected impact)
Working on this project is very interesting to me for several reasons: -It will allow me to learn about plant pathology in an environment that is perfectly suited for this purpose -It will give me experience with the model plant Arabidopsis which I plan to continue using after this position -Finally, in addition of mutations that will be analyzed in this work, the mutation machine will provide material that will be useful for future studies. All three aspects will useful to me later as I plan to continue investigating the mechanisms involved in disease resistance. Links with industry / industrial relevance (22)
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences genetics mutation
- medical and health sciences basic medicine pathology
- natural sciences biological sciences genetics genomes
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
NR47UH NORWICH
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.