Objective
Research objectives and content
The Rhizobium-legume symbiosis results in the formation of a new plant organ, the nitrogen-fixing root nodule. Nodule development is triggered by the Rhizobium lipochitooligosaccharide Nod factors. These bacterial signals induce likely more than one signalling pathway in the host plant roots. One of them results in the activation of the chalcone synthase (chs) gene that is at present the earliest gene induced by the Nod factor. The biological significance of chs activation in relation to nodulation is unknown. We will investigate the expression of chs under different conditions (Nod factors, plant hormones, wild type and mutant bacteria) in parallel to that of the early nodulins and cell cycle genes. These studies would clarify the relationship between chs activation and symbiotic signalling. The Nod factor-responsive chs cDNA will be identified and its corresponding genomic clone will be isolated. Promoter analysis described in the project might serve as basis for the identification of upstream elements required for the activation of Nod factor-responsive genes. Training content (objective, benefit and expected impact)
During my PhD Thesis I have acquired experience in standard techniques in microbiology and molecular genetics of bacteria. My postdoctoral stay at ISV would be very beneficial for my scientific formation because my training in molecular genetics would be expanded to higher organisms, as the plant partner of the Rhizobium-legume symbiosis. In addition, I would acquire expertise on techniques focused in RNA, promoter analyses and cloning and expression of eucariotic genes.
Links with industry / industrial relevance (22)
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences molecular biology molecular genetics
- natural sciences biological sciences microbiology bacteriology
- natural sciences biological sciences biological behavioural sciences ethology biological interactions
- natural sciences biological sciences genetics RNA
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Programme(s)
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
91190 GIF SUR YVETTE
France
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