Research objectives and content
Mammalian development requires both parental genomes for normal development. This his is due to imprinted genes whose expression is dependent on their parental origin. The mechanism of marking and subsequent regulation of expression of unprinted genes is unclear. The Hl 9 gene encodes an untranslated RNA transcribed from the matemal allele while the patenna allele is silent. Previous work indicates the presence of a 1.2kb silencer /imprinting element in the 5' region of the Hl9 gene. propose to carry out a detailed molecular aaalysis of tiliS control element. The aim is to identify the essentia'i sequences in the cis element for protein/DNA interaction, and to analyse the specific binding proteins and subsequent protein/protein interactions. This interaction must be a crucial step in the initaition of the unprint.
Training content (objective, benefit and expected impact)
To receive training in molecular developmental biology with spechal reference to epigeneitic modification involved in the imprinting phenomenon.Links with industry / industnal relevance (22) There is growing awareness t'aat epigenetic mechansims of gene regulation are involved in diseases and variations in susceptibility to diseases in indiividuals. The knowledge gained from this work therefore may eventually be crucial in aspects medicine as well as in biotechnology.