Objective
Research objectives and content
I shall analyse the actin binding properties, cellular distribution and function of ADF and cofilin, essential proteins involved in cytoskeletal remodelling, e.g. in cytokinesis and locomotion. Dr Weeds's laboratory and I are each well equipped to perform this work together. We have specific antibodies for ADF, cofilin and nuclear actin raised from three different animal sources. Preparing GFP-tagged proteins will permit us to investigate: (i) cellular distribution, response to stimuli and the role of two such similar proteins in one cell (ii) the role of phosphorylation and its regulation on intracellular localization and function of ADF/cofilin and (iii) the translocation of actin and cofilin to the nucleus. Using GFP-tags will enable us to monitor these effects in real time. We shall then be able to correlate biochemical evidence with in vivo studies which neither of us are equipped to do in isolation. Training Content
I have the cell biology and immunofluorescence expertise required by Dr Weeds and his group to perform in vivo analyses and in return they will instruct me further in biochemistry and the necessary molecular biology skills to prepare transfection, expression and mutated cofilin/ADF constructs.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences cell biology
- natural sciences biological sciences molecular biology
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
CB2 2QH Cambridge
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.