Objective
Research objectives and content
The overall aim of this project is to gain a thorough understanding of the molecular details of mismatch recognition by DNA repair enzymes. To do this, the interactions between E.coli MutS and synthetic DNA duplexes containing various mismatches and mutagenic bases will be estudied.The properties of the complete (96KDa) protein will be studied by a variety of biochemical techniques. In addition the structural details of MutS binding by preparing truncated derivatives (<30Kd) which retain mismatch binding activity will be examined. For this research programme, MutS protein and some regions of it will be synthesized and some techniques will be used to study the Interaction with mismatches in DNA: Footprinting, filter binding and gel retardation studies Ultraviolet melting (thermodynamic studies) Protein sequencing to determine the primary amino-acid sequence of MutS that binds to mismatches Crystallization of MutS and mini-proteins and their complexes with mismatches DNA
Training content (objective, benefit and expected impact)
This project will be a benefit for learning some of the techniques currently used in biochemistry assays. Having a better understanding of the features concerning the mismatches in DNA, could led to a closer knowledge of the causes and the therapy of cancer.
Links with industry / industrial relevance (22)
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics DNA
- medical and health sciences clinical medicine oncology
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
SO17 1BJ SOUTHAMPTON
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.